(VAOB200B) 200uL Sub Micro Absorption Cuvette, Bottom Black, Bonded, 2 Windows
$71.00
This sub-micro cuvette is for absorption applications, with a path length of 10 mm. For Aqueous Samples Only. Use up to 100°C. The price is for 1 piece.
- Type: Bonded, For Aqueous Samples Only
Description
VAOB200B Sub Micro Absorption Cuvette
The VAOB200B is a bottom-black bonded quartz cuvette engineered for UV/VIS absorption measurements with low-volume samples. Its 10 mm optical pathlength ensures compatibility with standard spectrophotometers, while the black bottom wall significantly reduces stray light and improves baseline stability — ideal for sensitive assays and trace-level analysis.
Key Features
- 200 µL working volume — designed for sub-micro assays with moderate sample volumes.
- 10 mm optical pathlength — fits most UV/VIS spectrophotometer holders.
- Bottom-black wall — minimizes stray light for enhanced signal-to-noise performance.
- 2 optical windows — optimized for direct absorbance measurement.
- Bonded fabrication — low cost and fast customization, best for aqueous or mild buffer solutions.
Typical Applications
- UV/VIS absorbance spectroscopy
- Protein, nucleic acid, and dye quantification
- Low-concentration and trace detection
- Enzyme kinetics and biochemical assays
Bonded Fabrication Notes
- Best suited for aqueous and mild buffer solutions.
- Not recommended for HF, strong acids/bases, strong organic solvents, or prolonged high-temperature use.
- Advantages: fast production, customizable design, and low cost.
- Typical optical path tolerance: ±0.06 – 0.08 mm.
- Bond seams may discolor with strongly staining samples; not intended for pressure sealing. For harsh solvents or long-term durability, consider fused or molded types.
Cuvette Specifications
| Type | Sub Micro Quartz Absorption Cuvette | Optical Path Length | 10 mm |
| Article No. | VAOB200B | Volume | 200 µL |
| Measuring Method | Absorption Cells | Wall Type | Bottom Black |
| Number of Windows | 2 | Wavelength Range | UV/VIS (190 – 2500 nm) |
| Height (Outside) | 45 mm | Aperture Window (Inside) | 2 × 10 mm |
| Width (Outside) | 12.5 mm | Depth (Inside) | 10 mm |
| Depth (Outside) | 12.5 mm | Center Height (Z) | 15 mm |
| Fabrication Method | Bonded* | Package | Cell & PTFE Lid |
Note: VAOB200B provides a cost-efficient solution for aqueous sample UV/VIS absorbance.
For more demanding applications requiring high solvent resistance or durability, fused or molded quartz cuvettes are recommended.
Comparison of Fabrication Types
| 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|
| *Molded | *Fused | *Bonded / Glued | |||
| UV vis ( 190- 2500 nm) | Yes | Yes | Yes | ||
| Transmission Matched | On Request | No | No | ||
| Transmission > 80% | Yes | Yes | Yes | ||
| Resistant to Acids and Bases (except hydrofluoric acid) | Yes | Yes | No | ||
| Resistant to Organic Solvents | Yes | Yes | No | ||
| Usable upto 600°C (1112°F) | Yes | Yes | Yes | ||
| Usable upto 1200°C (2192°F) | Yes | No | No | ||
| 0 Variations Reading | On Request | No | No | ||
| Fabrication | Molded | Assembled with Quartz Powder | Assembled with Glue | ||
| Adhesives | No | No | Yes | ||
| Storage | Short Term Storage | Clean After Use | Clean After Use | ||
| Available Material | Fused Quartz Material | ||||
| Note | Molded and fused cuvettes and cells are assembled by direct fusion without the use of adhesives throughout the production process. This guarantees resistance against corrosive chemicals and high temperature. Each quartz cuvette and cell has excellent transmission from 190nm to 2500nm. | ||||
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