When it comes to fluorescence applications, use QVARZ® cuvettes with four optically transparent windows. Our stock cuvettes are made of high-quality fused quartz and may be used to measure wavelengths ranging from 190 to 2500 nm. For high signal-to-noise ratios, this UV-vis transparent also has almost little autofluorescence. All common fluorometers can use cuvettes with path lengths ranging from 0.1 to 50 mm.
QVARZ® fluorescent cuvettes are composed of the highest quality raw materials, precisely constructed for maximum optical clarity, and have parallel walls of consistent thickness to maximize transmission and decrease refraction. Our molded cuvettes are mold-cavity sorted, assuring cuvette-to-cuvette uniformity and good reproducibility.
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About Measurement of Fluorescence
Fluorescence happens when a sample receives energy from electromagnetic radiation, generally UV light (photons). The energy “excites” the molecules, causing an atom’s electron to “kick” from a lower energy state to a higher energy excited state. The excited state has a relatively short lifespan (1-100 ns). When an electron returns to a lower energy state, the energy is released in the form of light (i.e., fluorescence). A monochromator filters some of the fluorescent light before it reaches the detector. The fluorescent light is emitted in all directions, however, to reduce interference, the detector is normally positioned at 90 degrees to the excitation light beam. As a result, four-sided transparent cuvettes are required for fluorescence applications.