Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength. This measurement can also be used to measure the amount
Why it’s a good idea to use self masking cuvettes… This article explains why it is important to use black walled cells for low volume work. We performed this experiment on a Varian Cary 500 a number of years ago to demonstrate this. The experiment: Take a suitable visible and stable chemcal, and make up
The primary difference between a cuvette and a test tube is that a cuvette has two straight sides whereas a test tube does not. Furthermore, cuvettes and test tubes serve distinct purposes and have different chemical compositions. In analytical chemistry, both the cuvette and the test tube are commonly used as analytical instruments. Both of
Colorimeter vs Spectrophotometer Colorimeter and spectrophotometer are the equipments used in colorimetry and spectrophotometry. Spectrophotometry and colorimetry are techniques, which can be used to identify the molecules depending on their absorption and emission properties. This is an easy technique to determine the concentration of a sample, which has a color. Though the molecule doesn’t have
UV – VIS absorption measurement cells or cuvettes as they are commonly called are glass or quartz rectangular cells which are used for holding the sample and reference solutions in beam path for making absorbance measurements. These are delicate items which require extreme care in handling to avoid breakage and at same time ensure accuracy
Hi everyone! We’re back with another exciting post about our favorite topic. Can you guess what it is? That’s right! Cuvettes!! We sure love to love cuvettes here at qvarz.com and do you know what we love even more? Providing you with the most complete information on cuvettes on the web. What is a Cuvette?
A spectrophotometer is ideal for determining how much a material absorbs or transmits a certain wavelength of light, resulting in concentration and purity information. In a normal research lab, a spectrophotometer includes a standard-sized chamber in which a cuvette is inserted, allowing a certain wavelength of light to travel a set distance through the sample, known as
Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions. It is based upon the interaction of light with the chemical bonds within a material. Raman is a light scattering technique, whereby a molecule scatters incident light from a high intensity laser light
Within molecular and biochemical applications, as well as in medical diagnostics, determination of the concentrations of substances in solution is a crucial analysis step. Frequently, photometric methods are employed for this purpose, as these can be conducted easily and quickly, and because they often represent the most cost-effective option. In general, (spectro)-photometric methods are based
It is a well-known fact that ordinary white light comprises of a mix of different wavelengths or colours. We are all familiar with the different colours of the rainbow which are in fact different colours comprising white light. White light is seldom used as such in spectroscopic analysis though of visible light source is a
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