(VAOB300B) 300uL Sub Micro Absorption Cuvette, Bottom Black, Bonded, 2 Windows
$71.00
This sub-micro cuvette is for absorption applications, with a path length of 10 mm. For Aqueous Samples Only. Use up to 100°C. The price is for 1 piece.
- Type: Bonded, For Aqueous Samples Only
Description
VAOB300B Sub Micro Absorption Cuvette
The VAOB300B is a bottom-black bonded quartz cuvette designed for sub-micro volume UV/VIS absorption measurements. With a 10 mm optical pathlength and a 300 µL sample capacity, this cuvette offers a balance between low sample consumption and enhanced light collection for sensitive assays. The black bottom wall effectively reduces stray light, improving signal stability and measurement accuracy.
Key Features
- 300 µL working volume — suitable for sub-micro absorbance measurements with higher sample stability.
- 10 mm optical pathlength — compatible with standard UV/VIS spectrophotometer holders.
- Bottom-black wall — reduces reflections and enhances signal-to-noise ratio in low-absorbance experiments.
- 2 optical windows — optimized for straight-path absorbance detection.
- Bonded fabrication — cost-efficient, with short lead time, best for aqueous and mild-buffer solutions.
Typical Applications
- UV/VIS absorbance spectroscopy
- Protein and nucleic acid quantification
- Low-concentration dye and biomolecule analysis
- Enzyme kinetics and biochemical assays
Bonded Fabrication Notes
- Recommended for aqueous or mild buffer media only.
- Not suitable for HF, strong acids/bases, strong organic solvents, or high-temperature soaking.
- Advantages: low cost, fast customization, short lead time.
- Typical optical path tolerance: ±0.06 – 0.08 mm.
- Bond seams may discolor with strongly staining samples; not intended for pressure sealing. For harsh solvents or long-term durability, consider fused or molded fabrication.
Cuvette Specifications
| Type | Sub Micro Quartz Absorption Cuvette | Optical Path Length | 10 mm |
| Article No. | VAOB300B | Volume | 300 µL |
| Measuring Method | Absorption Cells | Wall Type | Bottom Black |
| Number of Windows | 2 | Wavelength Range | UV/VIS (190 – 2500 nm) |
| Height (Outside) | 45 mm | Aperture Window (Inside) | 2 × 15 mm |
| Width (Outside) | 12.5 mm | Depth (Inside) | 10 mm |
| Depth (Outside) | 12.5 mm | Center Height (Z) | 15 mm |
| Fabrication Method | Bonded* | Package | Cell & PTFE Lid |
Note: VAOB300B is a cost-efficient solution for UV/VIS absorbance with moderate sample volume.
For applications requiring higher chemical resistance, durability, or solvent compatibility, consider fused or molded quartz alternatives.
Comparison of Fabrication Types
| 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|
| *Molded | *Fused | *Bonded / Glued | |||
| UV vis ( 190- 2500 nm) | Yes | Yes | Yes | ||
| Transmission Matched | On Request | No | No | ||
| Transmission > 80% | Yes | Yes | Yes | ||
| Resistant to Acids and Bases (except hydrofluoric acid) | Yes | Yes | No | ||
| Resistant to Organic Solvents | Yes | Yes | No | ||
| Usable upto 600°C (1112°F) | Yes | Yes | Yes | ||
| Usable upto 1200°C (2192°F) | Yes | No | No | ||
| 0 Variations Reading | On Request | No | No | ||
| Fabrication | Molded | Assembled with Quartz Powder | Assembled with Glue | ||
| Adhesives | No | No | Yes | ||
| Storage | Short Term Storage | Clean After Use | Clean After Use | ||
| Available Material | Fused Quartz Material | ||||
| Note | Molded and fused cuvettes and cells are assembled by direct fusion without the use of adhesives throughout the production process. This guarantees resistance against corrosive chemicals and high temperature. Each quartz cuvette and cell has excellent transmission from 190nm to 2500nm. | ||||
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