(VFOB100) New 100uL Sub Micro Fluorescence Cuvette, Black, Bonded, 4 Windows
$153.00
This sub-micro cuvette is for fluorescence applications, with a path length of 10 mm. For Aqueous Samples Only. Use up to 100°C. The price is for 1 piece.
- Type: Bonded, For Aqueous Samples Only
Description
VFOB100 Sub Micro Fluorescence Cuvette (4-Windows, All Black)
The VFOB100 is a sub-micro volume quartz fluorescence cuvette engineered for high-sensitivity fluorescence and absorbance applications with minimal sample volumes. With a 10 mm optical pathlength and only 100 µL volume, this cuvette is ideal for micro- and trace-level fluorescence measurements.
The 4-window design enables both excitation and emission collection at 90°, and the all-black wall structure minimizes stray light for maximum signal-to-noise performance.
Key Features
- 100 µL working volume — excellent for micro assays and trace analysis.
- 10 mm optical pathlength — compatible with most standard fluorometers and spectrophotometers.
- 4-window design — supports both excitation and emission detection for fluorescence measurements.
- All-black walls — effectively block stray light, improving fluorescence sensitivity and reducing background noise.
- Bonded fabrication — cost-effective and customizable; best suited for aqueous or mild buffer solutions.
- PTFE lid included — helps minimize evaporation during long or sensitive measurements.
Typical Applications
- Fluorescence and absorbance spectroscopy
- Low concentration biomolecule detection
- Enzyme kinetics and cell-free assays
- Trace analysis in life science and environmental testing
Bonded Fabrication Notes
- Best suited for aqueous and mild buffer solutions.
- Not recommended for HF, strong acids/bases, or strong organic solvents.
- Advantages: low cost, fast turnaround, easy customization.
- Optical path tolerance: ±0.06 – 0.08 mm.
- For high solvent resistance or harsh conditions, consider fused or molded cuvettes.
Cuvette Specifications
| Type | Sub Micro Quartz Fluorescence Cuvette | Optical Path Length | 10 mm |
| Article No. | VFOB100 | Volume | 100 µL |
| Measuring Method | Fluorescence Cells | Wall Type | All Black |
| Number of Windows | 4 | Wavelength Range | UV/VIS (190 – 2500 nm) |
| Height (Outside) | 45 mm | Aperture Window (Inside) | 2 × 5 mm |
| Width (Outside) | 12.5 mm | Depth (Inside) | 10 mm |
| Depth (Outside) | 12.5 mm | Center Height (Z) | 15 mm |
| Fabrication Method | Bonded* | Package | Cell & PTFE Lid |
Note: VFOB100 is designed specifically for micro-volume fluorescence detection, offering high sensitivity and low background.
For aggressive solvents or long-term durability, fused or molded cuvettes are recommended.
Comparison of Fabrication Types
| 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|
| *Molded | *Fused | *Bonded / Glued | |||
| UV vis ( 190- 2500 nm) | Yes | Yes | Yes | ||
| Transmission Matched | On Request | No | No | ||
| Transmission > 80% | Yes | Yes | Yes | ||
| Resistant to Acids and Bases (except hydrofluoric acid) | Yes | Yes | No | ||
| Resistant to Organic Solvents | Yes | Yes | No | ||
| Usable upto 600°C (1112°F) | Yes | Yes | Yes | ||
| Usable upto 1200°C (2192°F) | Yes | No | No | ||
| 0 Variations Reading | On Request | No | No | ||
| Fabrication | Molded | Assembled with Quartz Powder | Assembled with Glue | ||
| Adhesives | No | No | Yes | ||
| Storage | Short Term Storage | Clean After Use | Clean After Use | ||
| Available Material | Fused Quartz Material | ||||
| Note | Molded and fused cuvettes and cells are assembled by direct fusion without the use of adhesives throughout the production process. This guarantees resistance against corrosive chemicals and high temperature. Each quartz cuvette and cell has excellent transmission from 190nm to 2500nm. | ||||
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