Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions. It is based upon the interaction of light with the chemical bonds within a material. Raman is a light scattering technique, whereby a molecule scatters incident light from a high intensity laser light

Within molecular and biochemical applications, as well as in medical diagnostics, determination of the concentrations of substances in solution is a crucial analysis step. Frequently, photometric methods are employed for this purpose, as these can be conducted easily and quickly, and because they often represent the most cost-effective option. In general, (spectro)-photometric methods are based

It is a well-known fact that ordinary white light comprises of a mix of different wavelengths or colours. We are all familiar with the different colours of the rainbow which are in fact different colours comprising white light. White light is seldom used as such in spectroscopic analysis though of visible light source is a

Ideally the absorbance spectrum of a solution containing a single analyte should be a single absorption band at the wavelength of maximum absorbance. However, in real samples the spectrum gets influenced by presence of other interfering species. Such interferences can be easily eliminated by adopting different approaches covered in the article. Interferences in UV- Visible

Colorimetry and spectrophotometry vary in that colorimetry utilizes fixed wavelengths that are only observable in the visible spectrum, but spectrophotometry can use wavelengths from a larger range. We can utilize spectrophotometry and colorimetry to identify compounds based on their absorption and emission characteristics. Furthermore, this is a simple method for determining the concentration of a

Optical spectroscopic methods of analysis are based upon the interaction of electromagnetic radiation with matter. The objective of writing this article is to brief you on the fundamental concepts of commonly used spectroscopic techniques Electromagnetic Spectrum The electromagnetic spectrum provides the spectroscopist a wealth of information. Each region of the electromagnetic spectrum is characterised by

It is a well-known fact that ordinary white light comprises of a mix of different wavelengths or colours. We are all familiar with the different colours of the rainbow which are in fact different colours comprising white light. White light is seldom used as such in spectroscopic analysis though of visible light source is a

Many laboratories are subject to regulatory requirements that e.g. are prescribed by Quality Management Systems. These laboratories are thus obligated to test work equipment and analysis instruments, including (spectro-) photometers, on a regular basis. What if such regulations do not apply to your lab? In this case, testing is not absolutely necessary, as it is