(VAOB20B) 20uL Sub Micro Absorption Cuvette, Semi Black, Bonded, 2 Windows, Z dim 8.5mm
$139.00
This sub-micro cuvette is for absorption applications, with a path length of 10 mm. For Aqueous Samples Only. Use up to 100°C. The price is for 1 piece.
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- Type: Bonded, For Aqueous Samples Only
Description
VAOB20B Sub Micro Absorption Cuvette
The VAOB20B is a bottom-black bonded quartz cuvette designed for ultra-low-volume UV/VIS absorbance measurements. With a 10 mm optical pathlength and a 20 µL sample capacity, this cuvette is ideal for high-value or scarce samples where sensitivity and light control are critical. The black bottom wall reduces unwanted reflections and improves baseline stability for precise results.
Key Features
- 20 µL working volume — ultra-low sample requirement for high-value or limited-volume assays.
- 10 mm optical pathlength — compatible with standard UV/VIS spectrophotometers.
- Bottom-black wall — suppresses stray light and enhances S/N ratio.
- 2 optical windows — optimized for absorption detection.
- Z-dimension 8.5 mm — suitable for short-center-height instruments and plate-read configurations.
- Bonded fabrication — cost-effective and customizable, best for aqueous or mild buffer solutions.
Typical Applications
- UV/VIS absorbance with trace or valuable samples
- DNA, RNA, protein, or dye concentration analysis
- Enzyme kinetics in micro assays
- Low-signal measurements requiring controlled background
Bonded Fabrication Notes
- Best suited for aqueous and mild buffer solutions.
- Not suitable for HF, strong acids/bases, strong organic solvents, or prolonged high-temperature conditions.
- Advantages: quick customization, low production cost, short delivery time.
- Typical optical path tolerance: ±0.06 – 0.08 mm.
- Bond seams may discolor with strongly staining samples. For higher solvent resistance and durability, consider fused or molded cuvettes.
Cuvette Specifications
| Type | Sub Micro Quartz Absorption Cuvette | Optical Path Length | 10 mm |
| Article No. | VAOB20B | Volume | 20 µL |
| Measuring Method | Absorption Cells | Wall Type | Bottom Black |
| Number of Windows | 2 | Wavelength Range | UV/VIS (190 – 2500 nm) |
| Height (Outside) | 45 mm | Aperture Window (Inside) | 1 × 2 mm |
| Width (Outside) | 12.5 mm | Depth (Inside) | 10 mm |
| Depth (Outside) | 12.5 mm | Center Height (Z) | 8.5 mm |
| Fabrication Method | Bonded* | Package | Cell Only |
Note: VAOB20B is optimized for precise absorbance measurements in ultra-low volume settings.
For applications involving strong solvents or harsh conditions, fused or molded cuvettes are recommended.
Comparison of Fabrication Types
| 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|
| *Molded | *Fused | *Bonded / Glued | |||
| UV vis ( 190- 2500 nm) | Yes | Yes | Yes | ||
| Transmission Matched | On Request | No | No | ||
| Transmission > 80% | Yes | Yes | Yes | ||
| Resistant to Acids and Bases (except hydrofluoric acid) | Yes | Yes | No | ||
| Resistant to Organic Solvents | Yes | Yes | No | ||
| Usable upto 600°C (1112°F) | Yes | Yes | Yes | ||
| Usable upto 1200°C (2192°F) | Yes | No | No | ||
| 0 Variations Reading | On Request | No | No | ||
| Fabrication | Molded | Assembled with Quartz Powder | Assembled with Glue | ||
| Adhesives | No | No | Yes | ||
| Storage | Short Term Storage | Clean After Use | Clean After Use | ||
| Available Material | Fused Quartz Material | ||||
| Note | Molded and fused cuvettes and cells are assembled by direct fusion without the use of adhesives throughout the production process. This guarantees resistance against corrosive chemicals and high temperature. Each quartz cuvette and cell has excellent transmission from 190nm to 2500nm. | ||||
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