Within molecular and biochemical applications, as well as in medical diagnostics, determination of the concentrations of substances in solution is a crucial analysis step. Frequently, photometric methods are employed for this purpose, as these can be conducted easily and quickly, and because they often represent the most cost-effective option. In general, (spectro)-photometric methods are based

Spectrometer calibration accuracy is of critical importance for many optical characterization techniques such as Raman spectroscopy and interferometry.1–3 Typically, a calibration lamp is used for spectrometer calibration. Calibration lamps provide distinct, well-defined lines at a known wavelength, and these are assigned to the pixel indices of the detector. However, for small spectral ranges, where only a small number of calibration