{"id":68961,"date":"2022-02-21T12:48:11","date_gmt":"2022-02-21T04:48:11","guid":{"rendered":"https:\/\/qvarz.com\/cen-cuvette-an-ceann-ceart-gloine-vs-plaisteacha-vis-vs-uv-micrea-imleabhar-vs-macra-imleabhar\/"},"modified":"2022-02-21T12:48:47","modified_gmt":"2022-02-21T04:48:47","slug":"cen-cuvette-an-ceann-ceart-gloine-vs-plaisteacha-vis-vs-uv-micrea-imleabhar-vs-macra-imleabhar","status":"publish","type":"post","link":"https:\/\/qvarz.com\/ga\/cen-cuvette-an-ceann-ceart-gloine-vs-plaisteacha-vis-vs-uv-micrea-imleabhar-vs-macra-imleabhar\/","title":{"rendered":"C\u00e9n Cuvette An Ceann Ceart? Gloine vs Plaisteacha, VIS vs UV, Micrea-Imleabhar vs Macra-Imleabhar"},"content":{"rendered":"\n

Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a chur isteach i gcos\u00e1n solais f\u00f3taim\u00e9adar i bhform\u00e1id shainithe. Is iad Cuvettes, ie coime\u00e1d\u00e1in samplacha a bhfuil fuinneoga opt\u00fala iontu, an rogha chaighde\u00e1nach don fheidhmchl\u00e1r seo.<\/p>\n\n

Foinse \u00edomh\u00e1: science photo\/shutterstock.com<\/em>Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a chur isteach i gcos\u00e1n solais f\u00f3taim\u00e9adar i bhform\u00e1id shainithe. Is iad Cuvettes, ie coime\u00e1d\u00e1in samplacha a bhfuil fuinneoga opt\u00fala iontu, an rogha chaighde\u00e1nach don fheidhmchl\u00e1r seo. T\u00e1 an fad idir na fuinneoga opt\u00fala sainmh\u00ednithe go cruinn; ar an mbealach seo, t\u00e1 fad cos\u00e1n an tsampla taobh istigh den chuvette ar eolas. T\u00e1 roghn\u00fa na gcine\u00e1lacha \u00e9ags\u00fala cuvettes ollmh\u00f3r \u2013 fi\u00fa mura meastar ach na cuvettes sin a \u00fas\u00e1idtear le haghaidh tomhais ions\u00fach\u00e1in i r\u00e9imse na speictreasc\u00f3pachta UV-Vis. Is \u00e9 an cine\u00e1l cuvette is coitianta n\u00e1 cearnach, le tois\u00ed seachtracha 12.5 x 12.5 mm. Freastala\u00edonn an fhorm\u00e1id seo ar thoirteanna samplacha \u00f3n raon micril\u00edtear (cuvettes ultra-mhiocr\u00f3bach) go dt\u00ed an raon millil\u00edtear (macra cuvettes) (figi\u00far 1). Is \u00e9 10 mm fad cos\u00e1n caighde\u00e1nach cuvette; \u00e1fach, t\u00e1 cuvettes a shol\u00e1thra\u00edonn cos\u00e1n solais n\u00edos giorra tr\u00edd an sampla ar f\u00e1il freisin. Ina theannta sin, t\u00e1 difr\u00edocht idir cuvettes maidir lena n-\u00e1bhar, a n-airde agus m\u00e9id a bhfuinneog tomhais (figi\u00far 1).<\/p>\n\n

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F\u00edor 1: Cuvettes a bhfuil na tois\u00ed caighde\u00e1nacha seachtracha acu de 12.5 x 12.5 mm ach a dteasta\u00edonn \u00edosmh\u00e9ideanna samplacha \u00e9ags\u00fala uathu.<\/p>\n\n

Seice\u00e1il \u00e1r liosta Cuvettes Grianchloch le haghaidh Speictreasc\u00f3pacht UV\/Vis\/IR<\/a><\/p>\n\n

Beidh an cinneadh maidir le c\u00e9n cine\u00e1l cuvette a roghn\u00fa ag brath ar an ionstraim a \u00fas\u00e1idtear, ar n\u00e1d\u00far an iarratais agus ar air\u00edonna an tsampla. T\u00e1 s\u00e9 t\u00e1bhachtach go ginear\u00e1lta go mbeadh cuvettes chomh tr\u00e9dhearcach agus is f\u00e9idir chun na tonnfhaid a thomhas ionas nach gcuirfear teorainn le raon l\u00edneach an fh\u00f3taim\u00e9adar at\u00e1 ar f\u00e1il.\u00c9il\u00edonn roghn\u00fa an trealaimh ceanglais ar an cuvette toisc go gcaithfidh s\u00e9 a bheith ag lu\u00ed leis an bhfeiste. Baineann s\u00e9 seo go pr\u00edomha le tois\u00ed seachtracha an cuvette, toisc go gcaithfidh s\u00e9 a bheith oiri\u00fanach isteach sa seafta cuvette, ach t\u00e1 airde na bhfuinneoga tomhais r\u00edth\u00e1bhachtach freisin. Caithfidh siad seo a ail\u00edni\u00fa go foirfe leis an gcos\u00e1n solais a th\u00e9ann tr\u00edd an ionstraim. T\u00e1 an breithni\u00fa seo thar a bheith \u00e1bhartha maidir le cuvettes at\u00e1 deartha chun toirteanna beaga a thomhas agus a bhfuil fuinneoga tomhais an-bheag acu mar sin. Is iad airde coitianta na gcos\u00e1n solais 8.5 mm agus 15 mm.Baineann an ch\u00e9ad ghn\u00e9 th\u00e1bhachtach eile leis na tonnfhaid tomhais a bhaineann leis an iarratas at\u00e1 ar l\u00e1imh. N\u00edl cuvettes caighde\u00e1nach d\u00e9anta as PMMA, polaistir\u00e9in n\u00f3 gloine gn\u00e1th ach tr\u00e9dhearcach sa raon infheicthe. M\u00e1 \u00fas\u00e1idtear tonnfhaid sa raon UV, faoi bhun thart ar 300 nm, n\u00ed m\u00f3r cuvettes d\u00e9anta as gloine Grianchloch, n\u00f3 cine\u00e1l speisialta plaisteach, a shol\u00e1thra\u00edonn tr\u00e9dhearcacht leordh\u00f3thanach sa raon seo, a \u00fas\u00e1id (figi\u00far 2).<\/p>\n\n

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<\/figcaption><\/figure>\n

F\u00edor 2: Difr\u00edochta\u00ed idir speictream ions\u00fach\u00e1in cuvettes d\u00e9anta as \u00e1bhair \u00e9ags\u00fala, arna dtomhas idir 220 nm agus 400 nm<\/p>\n\n

T\u00e1 s\u00e9 r\u00edth\u00e1bhachtach sampla a th\u00e9amh agus a rial\u00fa go h\u00e9ifeachtach le linn an phr\u00f3isis tomhais do na modhanna sin a bh\u00edonn ag brath ar imoibrithe a tharla\u00edonn ag teocht shonrach \u00e1irithe agus a thomhaiseann ions\u00fa thar am. Chomh maith le leibh\u00e9al cu\u00ed friota\u00edochta an \u00e1bhair, t\u00e1 s\u00e9 t\u00e1bhachtach sa ch\u00e1s seo go bhfuil an limist\u00e9ar teagmh\u00e1la idir balla an cuvette agus an seafta cuvette rialaithe teochta chomh m\u00f3r agus is f\u00e9idir. Ar na c\u00faiseanna seo, cuireann cuvettes \u00e1irithe, mar shampla macra-cuvettes, bunt\u00e1iste ar f\u00e1il in iarratais at\u00e1 faoi rial\u00fa teochta.I measc na ngn\u00e9ithe eile a mbeidh tionchar acu ar rogha cuvette t\u00e1 n\u00e1d\u00far, toirt agus ti\u00fachan an tsampla at\u00e1 ar l\u00e1imh.M\u00e1 t\u00e1 an sampla bunaithe ar thuaslag\u00e1n uisc\u00ed, t\u00e1 an t-\u00e1bhar as a nd\u00e9antar an cuvette s\u00e1ch neamhlean\u00fanach. M\u00e1s rud \u00e9, ar an l\u00e1imh eile, go bhfuil tuaslag\u00f3ir\u00ed org\u00e1nacha i gceist, is iad cuvettes gloine an rogha is fearr mar go l\u00e9ir\u00edonn siad seo friota\u00edocht n\u00edos airde i gcompar\u00e1id le malairt\u00ed d\u00e9anta as plaisteach.Mura bhfuil ach m\u00e9id beag sampla ar f\u00e1il, d\u2019fh\u00e9adfa\u00ed smaoineamh ar an sampla a ath\u00fas\u00e1id chun na tomhais seo a leanas a dh\u00e9anamh. Sa ch\u00e1s seo, moltar cuvettes plaisteacha aon\u00fas\u00e1ide. M\u00e1 dh\u00e9antar cuvettes plaisteacha a phac\u00e1isti\u00fa ina n-aonar agus de ghr\u00e1d \u00edonachta cu\u00ed, laghd\u00f3far an baol \u00e9illithe. De rogha air sin, f\u00e9adfar cuvettes a roghn\u00fa a dearadh chun freastal ar l\u00edon f\u00edorbheag.Beidh tionchar ag ti\u00fachan an tsampla freisin ar an rogha cuvette toisc go bhfuil uasteorainn braite ag gach ionstraim. Mar shampla, m\u00e1 \u00fas\u00e1idtear f\u00f3taim\u00e9adar le raon tomhais l\u00edneach suas le 2 A le fad cos\u00e1in 10 mm, is f\u00e9idir DNA dh\u00e1 shn\u00e1ithe a chainn\u00edocht\u00fa go hiontaofa suas go dt\u00ed uasti\u00fachan 100 \u00b5g\/ml. N\u00ed m\u00f3r tuaslag\u00e1in de thi\u00fachan n\u00edos airde a chaol\u00fa, n\u00f3 is f\u00e9idir an caol\u00fa a insamhladh le cuvette a bhfuil fad cos\u00e1in n\u00edos giorra aige. De r\u00e9ir dhl\u00ed Lambert-Beer, ceada\u00edonn fad cos\u00e1in 1 mm comhchruinnithe dsDNA chomh hard le 1,000 \u00b5g\/mL a thomhas.Mura bhfuil s\u00e9 forordaithe ag n\u00e1d\u00far an iarratais, cuireann \u00e1bhar an chuvette rogha eile le d\u00e9anamh. Go ginear\u00e1lta, l\u00e9ir\u00edonn cuvettes gloine n\u00edos m\u00f3 tr\u00e9dhearcachta agus cruinneas tomhais, agus is f\u00e9idir iad a ath\u00fas\u00e1id go minic. Ansin ar\u00eds, t\u00e1 l\u00e1imhse\u00e1il cuvettes plaisteach simpl\u00ed agus s\u00e1bh\u00e1ilte. \u00d3s rud \u00e9 nach n-\u00fas\u00e1idtear cuvettes plaisteacha ach uair amh\u00e1in agus nach dteasta\u00edonn glanadh orthu, n\u00ed g\u00e1 dam\u00e1iste agus caillteanas f\u00e9ideartha a chur san \u00e1ireamh. <\/p>\n\n

Foinse<\/a><\/p>\n\n

<\/p>\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a chur isteach i gcos\u00e1n solais f\u00f3taim\u00e9adar i bhform\u00e1id shainithe. Is iad Cuvettes, ie coime\u00e1d\u00e1in samplacha a bhfuil fuinneoga opt\u00fala iontu, an rogha chaighde\u00e1nach don fheidhmchl\u00e1r seo. Foinse \u00edomh\u00e1: science photo\/shutterstock.com Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a chur<\/p>\n

Read More<\/a><\/div>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","ub_ctt_via":"","footnotes":""},"categories":[842,830],"tags":[],"class_list":["post-68961","post","type-post","status-publish","format-standard","hentry","category-cuvettes-agus-cealla","category-gach-post"],"featured_image_src":null,"author_info":{"display_name":"admin","author_link":"https:\/\/qvarz.com\/ga\/author\/admin\/"},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"bacola-woo-product":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false,"variation_swatches_image_size":false,"variation_swatches_tooltip_size":false,"dgwt-wcas-product-suggestion":false},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/qvarz.com\/ga\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a chur isteach i gcos\u00e1n solais f\u00f3taim\u00e9adar i bhform\u00e1id shainithe. Is iad Cuvettes, ie coime\u00e1d\u00e1in samplacha a bhfuil fuinneoga opt\u00fala iontu, an rogha chaighde\u00e1nach don fheidhmchl\u00e1r seo. Foinse \u00edomh\u00e1: science photo\/shutterstock.com Chun cr\u00edche anail\u00eds\u00ed f\u00f3taim\u00e9adracha ar shampla\u00ed leachtacha, n\u00ed m\u00f3r an r\u00e9iteach a churRead…","_links":{"self":[{"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/posts\/68961","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/comments?post=68961"}],"version-history":[{"count":1,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/posts\/68961\/revisions"}],"predecessor-version":[{"id":68963,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/posts\/68961\/revisions\/68963"}],"wp:attachment":[{"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/media?parent=68961"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/categories?post=68961"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/qvarz.com\/ga\/wp-json\/wp\/v2\/tags?post=68961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}